hydroxyapatite beads Search Results


hydroxyapatite bead ( average diameter)  (Berkeley Advanced Biomaterials)
90
Berkeley Advanced Biomaterials hydroxyapatite bead ( average diameter)
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Hydroxyapatite Bead ( Average Diameter), supplied by Berkeley Advanced Biomaterials, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite beads central glass co., tokyo  (Central Glass Co Ltd)
90
Central Glass Co Ltd hydroxyapatite beads central glass co., tokyo
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Hydroxyapatite Beads Central Glass Co., Tokyo, supplied by Central Glass Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hydroxyapatite beads central glass co., tokyo/product/Central Glass Co Ltd
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hydroxyapatite beads central glass co., tokyo - by Bioz Stars, 2026-06
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saliva-coated hydroxyapatite beads  (BDH Chemicals Ltd)
90
BDH Chemicals Ltd saliva-coated hydroxyapatite beads
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Saliva Coated Hydroxyapatite Beads, supplied by BDH Chemicals Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/saliva-coated hydroxyapatite beads/product/BDH Chemicals Ltd
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saliva-coated hydroxyapatite beads - by Bioz Stars, 2026-06
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hydroxyapatite beads  (BDH Chemicals Ltd)
90
BDH Chemicals Ltd hydroxyapatite beads
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Hydroxyapatite Beads, supplied by BDH Chemicals Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hydroxyapatite beads/product/BDH Chemicals Ltd
Average 90 stars, based on 1 article reviews
hydroxyapatite beads - by Bioz Stars, 2026-06
90/100 stars
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spheroidal hydroxyapatite beads  (Seikagaku corporation)
90
Seikagaku corporation spheroidal hydroxyapatite beads
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Spheroidal Hydroxyapatite Beads, supplied by Seikagaku corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spheroidal hydroxyapatite beads/product/Seikagaku corporation
Average 90 stars, based on 1 article reviews
spheroidal hydroxyapatite beads - by Bioz Stars, 2026-06
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porous hydroxyapatite beads  (Clarkson Chromatography Products)
90
Clarkson Chromatography Products porous hydroxyapatite beads
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Porous Hydroxyapatite Beads, supplied by Clarkson Chromatography Products, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/porous hydroxyapatite beads/product/Clarkson Chromatography Products
Average 90 stars, based on 1 article reviews
porous hydroxyapatite beads - by Bioz Stars, 2026-06
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hydroxyapatite beads  (Corning Life Sciences)
90
Corning Life Sciences hydroxyapatite beads
MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to <t>hydroxyapatite</t> (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.
Hydroxyapatite Beads, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hydroxyapatite beads/product/Corning Life Sciences
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hydroxyapatite beads - by Bioz Stars, 2026-06
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Image Search Results


MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.

Journal: Bone

Article Title: Polyphosphates inhibit extracellular matrix mineralization in MC3T3-E1 osteoblast cultures

doi: 10.1016/j.bone.2013.01.020

Figure Lengend Snippet: MC3T3-E1 osteoblasts were cultured in osteogenic media and treated with either 10 μM PolyP5 or PolyP65 for the indicated 2-day intervals. After 12 days of culture, mineral was stained in the plates (A) by the von Kossa method (black), or (B) the cell/matrix layer was embedded in LR White acrylic resin, sectioned perpendicular to the culture dish plane, and sections were stained with the von Kossa method followed by counterstaining with toluidine blue. By both methods, less mineral is seen after PolyP treatment starting at days 4–6 when the matrix starts to mineralize; earlier treatments when matrix is being first secreted and assembled had little effect. (C) Direct binding of PolyPs to hydroxyapatite (HAP) was determined by incubating the indicated concentrations of synthetic HAP beads in either 0.5 mM PolyP5 or PolyP65 solutions, and quantifying the remaining concentration of PolyP in the supernatant (by acid hydrolysis and phosphate quantification). Increasing amounts of HAP clearly leads to increasing depletion of PolyPs from solution. Data are presented as mean values ± SE. *p<0.05, **p<0.01, ***p<0.001 Student's t-test relative to 0 mg/ml HAP. (D) When 20 μg PolyP are bound to 2 mg HAP, hydrolysis of the polyphosphates by TNAP is less than that of polyphosphate alone. Adsorption of polyphosphate onto HAP was verified by supernatant acid hydrolysis followed by phosphate quantification. Data are presented as mean values ± SE. **p<0.01, ***p<0.001 Student's t-test relative to free PolyP.

Article Snippet: Hydroxyapatite binding assay A 20 mg/ml stock hydroxyapatite bead (5 μm average diameter, Berkeley Advanced Biomaterials, Berkeley, CA, USA) slurry was generated by washing the beads as received from the supplier three times in Tris-buffered saline, pH 7.0 (TBS), and then reconstituting in TBS.

Techniques: Cell Culture, Staining, Binding Assay, Concentration Assay, Adsorption